Journal of the Indian Medical Association May 1953

But in specimens subjected to the action of ribonuclease in the fresh state (vide supra) the nucleus showed a paler colour and in some of the flagellates the darker staining karyosome and the peripheral ring of the nucleus was replaced by a nuber of dark short dot or rod like structures (Plate I Figs. [...] Our study of the nucleotides in L. donovani shows that DNA is present in the nucleus and the parabasal body of the parasite in both the leishmania and the leptomonad forms. [...] The Feuigen method is a specific reaction for DNA and thus the appearance of the nucleus of the leptomonad as seen with this method represents the true picture and there is DNA in the nuclear membrane of the leptornonad as in the leishmania form. [...] The fact that the parabasal body lying on the dorsal wall of the flagellar vacuole and usually occuping a posterior position contains DNA may be held as evidence in support of the view that these two together represent the second nuclear structure in the protozoon the flagellar vacuole being the nuclear sac and the parabasal body the chromatinic structure (c.f. [...] The distribution of DNA in the nucleus is described; only the karyosome and the periphery of the nucleus of both the leishmania and the leptomonad forms show the presence of DNA.